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Paul, 26, went the full distance against Floyd Mayweather in their eight-round exhibition bout at the Hard Rock Stadium in Miami, Florida, last month.
The American YouTuber, who secured a lucrative payday against Mayweather, has previously claimed that he could beat Tyson in a fight.
KSI handed Paul his first professional boxing defeat in their blockbuster 2019 clash at the Staples Center in Los Angeles.
The 28-year-old YouTuber and rapper has brushed aside his rivalry with Paul by claiming that the American could beat Iron Mike.
KSI argued that "people don't understand that we train like boxers" and Tyson is no longer the young, ferocious fighter he was during his prime.
Speaking on Paul's Impaulsive podcast, he said: "Bro, with you and Tyson, I think you could beat Tyson.
"I know people are going to be like, 'Oh, what the f**k, JJ?' But, bro, you've got to remember that this isn't Tyson who's 18, this is Tyson who's 55.
"He's a legend, that's the thing, but people don't understand that we train like boxers, man, because we're going in there with the world watching.
"Everyone is saying we can't do this, we can't do that, but do you think we're going in there like, 'Oh, we're YouTubers, we should try and just wing it?'
"No way, bro, winging it is kind of what Deji [Olatunji] did, he thought he could get away with doing the bare minimum.
"He thought, 'I'm kind of strong, I've kind of got some technique, I should be able to just do it,' but bro, we go in and we are gladiators.
"We have to prove to everyone that we can fight, and we're going to f*****g do it, and nobody can say s**t and when we do it people go, 'Oh, we didn't expect that,' but come on, man, we're YouTubers that's how we are."
Paul was recently asked if he was "ready to fight" Tyson, with the YouTuber remaining cryptic about his future in boxing.
However, a leaked Instagram message has hinted that he could make his sensational boxing return against UFC legend Anderson Silva.
Featured Image Credit: Impaulsive/Hotboxin' with Mike Tyson
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